BioNano activities

Super-resolution imaging of nuclear organization

Cell Biophysics & Physical Biology

Project description

DNA is packaged inside the nucleus in the form of chromatin. DNA wraps around an octamer of histone proteins in two-turns, forming the nucleosome, the repeating unit of the chromatin. Further compaction is facilitated by linker histones and folding of the chromatin fiber into higher order structures. While the organization of chromatin inside the nucleus is highly important for its function in gene expression and in determining cellular phenotype, visualizing this organization is extremely challenging in the cellular context. We are developing quantitative super-resolution microscopy methods to overcome this limitation and study the intimate link between chromatin structure and its function in diverse cellular processes.

External collaborations

Maria Pia Cosma (CRG), Manuel Mendoza (CRG), Jerome Solon (CRG), Rafael Casellas (NIH)

Selected publications in this project

News

  • ICFO in Cell

    Super-resolution microscopes reveal the link between Genome Packaging and Cell Pluripotency. Until now it was known that our DNA is packaged by regular repeating units of the nucleosomes throughout the genome which give rise to chromatin. However, due to the lack of suitable techniques and instruments, the chromatin organization inside a cell nucleus could not … Continue reading →

Funding

Present Spanish Misnistry of Education – Explora Ingenio (for radically new ideas)

Future and Emerging Technologies FET-Open, CellViewer ERC-MOTORS (ERC-Starting Grant)

▲ Super-resolution imaging of nuclear organization