BioNano activities

Quantitative strategies for SR and single molecule dynamics

Development of nano-tools for quantitative bio-imaging

Project description

Over the last ten years, enormous developments in imaging technologies, quantitative analysis algorithms, new optical probes and breakthrough genetic engineering methods have enabled the rapid emergence and application of novel single molecule fluorescence techniques, such as single particle tracking and super-resolution nanoscopy. While these approaches are extremely powerful in Biology, there is an increasing demand for robust, unbiased quantification methods of the raw data. We are developing different analysis algorithms that provide detailed quantification of multicolour single particle tracking data to reveal diffusion patterns and dynamic interactions between individual molecules in living cells. We also develop different analysis algorithms for unbiased quantification of STED and single molecule localization-based super-resolution images.

External collaborations

Selected publications in this project

News

  • ICFO on the cover of Chemistry Select

    Single molecule fluorescence reveals dimerization of the oncogene Src. The tyrosine-protein kinase Src is a key element of signaling cascades involved in the invasive and metastasis-forming capacity of cancer cells. Researchers Bruno Castro and Erik Garbacik working in the Single Molecule Biophotonics group led by ICREA Professor at ICFO Maria Garcia-Parajo in collaboration with the … Continue reading →

  • ICFO in Reports of Progress in Physics

    ICFO publishes review of progress in single particle tracking. Single particle tracking is a powerful optical approach to study a variety of dynamic processes in life sciences. A recent review written by Carlo Manzo and ICREA Professor at ICFO Maria Garcia-Parajo published in Reports of Progress in Physics discusses the principles of single particle tracking … Continue reading →

  • ICFO in Cell

    Super-resolution microscopes reveal the link between Genome Packaging and Cell Pluripotency. Until now it was known that our DNA is packaged by regular repeating units of the nucleosomes throughout the genome which give rise to chromatin. However, due to the lack of suitable techniques and instruments, the chromatin organization inside a cell nucleus could not … Continue reading →

  • Role of disorder in cell membranes and connection with function

    Weak ergodicity breaking of receptor motion in living cells stemming from random diffusivity in PRX. Fundamental biological processes, including the capture of pathogens by membrane receptors, are regulated by molecular transport. A joint collaboration between the ICREA Professors at ICFO Maria Garcia-Parajo and Maciej Lewenstein show that receptor functioning is linked to nonergodic dynamics, which … Continue reading →

  • Two recent ICFO papers highlighted by the HFSP

    PNAS and J. Cell Science papers highlighted for new findings in Biology by means of super-resolution and single molecules approaches. Two recent studies published in PNAS and J. cell Science by the Single Molecule Biophotonics group led by ICREA Professor at ICFO Maria Garcia-Parajo have been highlighted by the Human Frontiers Science Program (HFSP) for … Continue reading →

  • ICFO paper highlighted by The Faculty of 1000

    PNAS publication cited for new findings in biology by means of technically advanced imaging instrumentation. The recent study published in PNAS by the Single Molecule Biophotonics group led by ICREA Professor at ICFO Maria Garcia-Parajo has been recommended by The Faculty of 1000 as an important paper that provides new findings in biology by means … Continue reading →

  • The advantages of order in Biology

    Cell membranes exploit hierarchical order to efficiently internalize pathogens in PNAS. A recent study carried out by Juan Torreno-Pina, Bruno Castro and Carlo Manzo of the Single Molecule Biophotonics group led by ICREA Professor at ICFO María García-Parajo, established a crucial role for the organization of the cell membrane in the recognition and binding of … Continue reading →

Funding

HFSP advanced grant; Fundacio Cellex, MICINN– Plan Nacional MAT2011-22887: IMMU-NANO-FORCE; MINECO-FIS2014-56107-R

▲ Quantitative strategies for SR and single molecule dynamics