Dynamic nanolandscape of immune receptors
Cell Biophysics & Physical BiologyProject description
Our aim is to exploit state-of-the-art optical nanoscopy to unravel the nanolandscape of cells of the immune system and get dynamic information of the cell surface organization of cellular receptors and subsequent signalling pathways at the nanometer level. In particularly in the immune system, which defends our body against pathogens, recognition and elimination of nanometer-sized pathogens is regulated through complex cell signaling and cell communication to ensure a swift and effective protective response. Understanding how these cell surface processes are orchestrated at the molecular level on living cells is at the core of this project.
ICFO groups associated with the project
ICFO publications associated with the project
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Changes in membrane sphingolipid composition modulate dynamics and adhesion of integrin nanoclusters
C. Eich, C. Manzo, S.D. Keijzer, G. Bakker, I. Reinieren-Beeren, M.F. García-Parajo, and A. Cambi,Scientific Reports, vol. 6, 2016.
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Weak Ergodicity Breaking of Receptor Motion in Living Cells Stemming from Random Diffusivity
C. Manzo, J.A. Torreno-Pina, P. Massignan, G.J. Lapeyre, M. Lewenstein, and M.F. Garcia Parajo,Physical Review X, vol. 5, 2015.
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Nanoclustering as a dominant feature of plasma membrane organization
M.F. Garcia-Parajo, A. Cambi, J.A. Torreno-Pina, N. Thompson, and K. Jacobson,Journal of Cell Science, vol. 127, pp. 4995-5005, 2014.
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Priming by Chemokines Restricts Lateral Mobility of the Adhesion Receptor LFA-1 and Restores Adhesion to ICAM-1 Nano-Aggregates on Human Mature Dendritic Cells
K.J.E. Borgman, T.S. van Zanten, C. Manzo, R. Cabezón, A. Cambi, D. Benítez-Ribas, and M.F. Garcia-Parajo,PLoS ONE, vol. 9, pp. e99589, 2014.
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Meeting Report – Visualizing signaling nanoplatforms at a higher spatiotemporal resolution
A. Cambi, M. Lakadamyali, D.S. Lidke, and M.F. Garcia-Parajo,Journal of Cell Science, vol. 126, pp. 3817-3821, 2013.
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Lateral mobility of individual integrin nanoclusters orchestrates the onset for leukocyte adhesion
G.J. Bakker, C. Eich, J.A. Torreno-Pina, R. Diez-Ahedo, G. Perez-Samper, T.S. van Zanten, C.G. Figdor, A. Cambi, and M.F. Garcia-Parajo,Proceedings of the National Academy of Sciences, vol. 109, pp. 4869-4874, 2012.
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The Neck Region of the C-type Lectin DC-SIGN Regulates Its Surface Spatiotemporal Organization and Virus-binding Capacity on Antigen-presenting Cells
C. Manzo, J.A. Torreno-Pina, B. Joosten, I. Reinieren-Beeren, E.J. Gualda, P. Loza-Alvarez, C.G. Figdor, M.F. Garcia-Parajo, and A. Cambi,Journal of Biological Chemistry, vol. 287, pp. 38946-38955, 2012.
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Dynamic Imaging of Cell‐Free and Cell‐Associated Viral Capture in Mature Dendritic Cells
N. Izquierdo‐Useros, O. Esteban, M.T. Rodriguez‐Plata, I. Erkizia, J.G. Prado, J. Blanco, M.F. García‐Parajo, and J. Martinez‐Picado,Traffic, vol. 12, pp. 1702-1713, 2011.
External collaborations
Dr. Alessandra Cambi (NCMLS, Nijmegen, Netherlands); Prof. Enzo Curundolo (Weatherall Institute of Molecular Medicine, University of Oxford, UK); Prof. Javier Martinez-Picado (IrsiCaixa, Badalona, Spain); Dr. Daniel Benitez-Ribas (Hospital Clinic Barcelona, Spain)
Funding
HFSP advanced grant; MINECO Severo Ochoa, Fundacio Privada CELLEX Barcelona, MINECO-FIS2014-56107-R.
