BioNano activities

Development of quantitative, high spatiotemporal resolution imaging methods

Development of nano-tools for quantitative bio-imaging

Project description

Super-resolution microscopy has revolutionized light microscopy, pushing the spatial resolution well below the diffraction limit. The impact of super-resolution methods have already been recognized by the Nobel Prize in Chemistry in 2014. Important challenges remain in pushing the application of these methods in biology. One of these limitations is the ability to extract quantitative information from the images, which is confounded by fluorophore photophysics. In addition, the temporal resolution is also limited by fluorophore photostability and photoswitching characteristics. We are developing methods to circumvent these limitations and extend the spatiotemporal resolution and quantitative power of super-resolution microscopy.

External collaborations

Selected publications in this project


  • ICFO in Reports of Progress in Physics

    ICFO publishes review of progress in single particle tracking. Single particle tracking is a powerful optical approach to study a variety of dynamic processes in life sciences. A recent review written by Carlo Manzo and ICREA Professor at ICFO Maria Garcia-Parajo published in Reports of Progress in Physics discusses the principles of single particle tracking … Continue reading →

  • ICFO in Cell

    Super-resolution microscopes reveal the link between Genome Packaging and Cell Pluripotency. Until now it was known that our DNA is packaged by regular repeating units of the nucleosomes throughout the genome which give rise to chromatin. However, due to the lack of suitable techniques and instruments, the chromatin organization inside a cell nucleus could not … Continue reading →

  • Measuring Photoactivation Efficiency at the single molecule level

    ICFO’s Advanced fluorescence imaging and biophysics group in Nature Methods. The ability to determine protein stoichiometry and monitor changes in the balance between monomeric, dimeric and multi-meric proteins allows scientists to see the difference between a properly functioning cell and a diseased cell. For this reason, there is a great interest in being able to … Continue reading →

  • ICFO in the Systems Microscopy Consortium

    The group led by Prof. Melike Lakadamyali joins a live cell imaging network of excellence. The group led by Nest Fellow Prof. Melike Lakadamyali will be a new partner in the Systems Microscopy Network of Excellence. This multidisciplinary consortium coordinated by Karolinska Institutet (Sweden) unites 15 European research groups. The project was launched on 1 … Continue reading →


Present ERC-MOTORS (ERC-Starting Grant), FP-7- HEALTH.2010. (Systems Microscopy Network of Excellence), Future and Emerging Technologies, FET-Open, CellViewer

Past Spanish Ministry of Education, Plan Nacional, Marie Curie International Re-Integration Grant, ICFO-Startup Package

▲ Development of quantitative, high spatiotemporal resolution imaging methods